Public documents
This section provides documentation of interest in the field of marine biotoxins.
EU legislation
COMMISSION DELEGATED REGULATION (EU) 2019/478 of 14 January 2019 amending Regulation (EU) 2017/625 of the European Parliament and of the Council as regards the categories of consignments to be subjected to official controls at border control posts.
COMMISSION DELEGATED REGULATION (EU) 2019/2127 of 10 October 2019 amending Regulation (EU) 2017/625 of the European Parliament and of the Council as regards the date of application of certain provisions of Council Directives 91/496/EEC, 97/78/EC and 2000/29/EC.
REGULATION (EU) 2021/1756 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 6 October 2021 amending Regulation (EU) 2017/625 as regards official controls on animals and products of animal origin exported from third countries to the Union in order to ensure compliance with the prohibition of certain uses of antimicrobials and Regulation (EC) No 853/2004 as regards the direct supply of meat from poultry and lagomorphs.
Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official controls and other official activities performed to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant protection products, amending Regulations (EC) No 999/2001, (EC) No 396/2005, (EC) No 1069/2009, (EC) No 1107/2009, (EU) No 1151/2012, (EU) No 652/2014, (EU) 2016/429 and (EU) 2016/2031 of the European Parliament and of the Council, Council Regulations (EC) No 1/2005 and (EC) No 1099/2009 and Council Directives 98/58/EC, 1999/74/EC, 2007/43/EC, 2008/119/EC and 2008/120/EC, and repealing Regulations (EC) No 854/2004 and (EC) No 882/2004 of the European Parliament and of the Council, Council Directives 89/608/EEC, 89/662/EEC, 90/425/EEC, 91/496/EEC, 96/23/EC, 96/93/EC and 97/78/EC and Council Decision 92/438/EEC (Official Controls Regulation).
REGULATION (EC) No 852/2004 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 29 April 2004 on the hygiene of foodstuffs.
- Corrigendum to Regulation (EC) No 852/2004 of the European Parliament and of the Council of 29 April 2004 on the hygiene of foodstuffs
- COMMISSION REGULATION (EC) No 1019/2008 of 17 October 2008 amending Annex II to Regulation (EC) No 852/2004 off the European Parliament and of the Council on the hygiene of foodstuffs
COMMISSION REGULATION (EC) No 2074/2005 of 5 December 2005 laying down in implementing measures for certain products under Regulation (EC) nº 853/2004 of the European Parliament and of the Council and for the organisation of official controls under Regulation (EC) nº 854/2004 of the European Parliament and of the council and Regulation (EC) nº 882/2004 of the European Parliament and of the Council, derogating from Regulation (EC) nº 852/2004 of the European Parliament and of the Council and amending Regulations (EC) nº 853/2004 and (EC) nº 854/2004.
- COMMISSION REGULATION (EC) No 1664/2006 of 6 November 2006 amending Regulation (EC) No 2074/2005 as regards implementing measures for certain products of animal origin intended for human consumption and repealing certain implementing measures
- COMMISSION REGULATION (EC) No 1244/2007 of 24 October 2007 amending Regulation (EC) No 2074/2005 as regards implementing measures for certain products of animal origin intended for human consumption and laying down specific rules on official controls for the inspection of meat
- COMMISSION REGULATION (EU) No 15/2011 of 10 January 2011 amending Regulation (EC) No 2074/2005 as regards recognised testing methods for detecting marine biotoxins in live bivalve molluscs
- COMMISSION REGULATION (EU) 2017/1980 of 31 October 2017 amending Annex III to Regulation (EC) No 2074/2005 as regards paralytic shellfish poison (PSP) detection method
- COMMISSION IMPLEMENTING REGULATION (EU) 2019/1139 of 3 July 2019 amending Regulation (EC) No 2074/2005 as regards official controls on food of animal origin in relation to requirements concerning food chain information and fishery products and to the reference to recognised testing methods for marine biotoxins and to testing methods for raw milk and heat-treated cow's milk
COMMISSION IMPLEMENTING REGULATION (EU) 2019/627 of 15 March 2019 laying down uniform practical arrangements for the performance of official controls on products of animal origin intended for human consumption in accordance with Regulation (EU) 2017/625 of the European Parliament and of the Council and amending Commission Regulation (EC) No 2074/2005 as regards official controls
- COMMISSION IMPLEMENTING REGULATION (EU) 2021/1709 of 23 September 2021 amending Implementing Regulation (EU) 2019/627 as regards uniform practical arrangements for the performance of official controls on products of animal origin
REGULATION (EC) No 853/2004 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 29 April 2004 laying down specific hygiene rules for food of animal origin
- Corrigendum to Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin
- COMMISSION REGULATION (EU) No 558/2010 of 24 June 2010 amending Annex III to Regulation (EC) No 853/2004 of the European Parliament and of the Council laying down specific hygiene rules for food of animal origin
- COMMISSION REGULATION (EU) No 786/2013 of 16 August 2013 amending Annex III to Regulation (EC) No 853/2004 of the European Parliament and of the Council as regards the permitted limits of yessotoxins in live bivalve molluscs
- COMMISSION REGULATION (EU) 2017/1978 of 31 October 2017 amending Annex III to Regulation (EC) No 853/2004 of the European Parliament and of the Council laying down specific hygiene rules for food of animal origin as regard echinoderms harvested outside classified production areas
- REGULATIONS COMMISSION DELEGATED REGULATION (EU) 2021/1374 of 12 April 2021 amending Annex III to Regulation (EC) No 853/2004 of the European Parliament and of the Council on specific hygiene requirements for food of animal origin
Annual Work Program
The Annual Work Program defines the strategic lines of action, technical activities, and coordination tasks that the EURLMB must carry out, in accordance with the mandate conferred by EU legislation and in support of the NRL network.
Activities of the EU Reference Laboratories and EU Reference Centres in 2025-2027
WP-EURLMB-2023-2024 (January 2023-December 2024)
WP-EURLMB-2021-2022 (January-December 2021-2022)
WP-EURLMB-2020 (January-December 2020-2021)
WP-EURLMB-2019 (January-December 2019-2020)
WP-EURLMB-2018 (January-December 2018)
WP-EURLMB-2016-2017 (January 2016-December 2017)
WP-EURLMB-2015 (January-December 2015)
WP-EURLMB-2014 (January-December 2014)
WP-EURLMB-2013 (January-December 2013)
WP-EURLMB-2012 (16/02/2012)
WP-EURLMB-2011 (19/04/2011)
WP-CRLMB-2010 (19/04/2010)
Scientific support
Access here the most relevant scientific publications in the field of marine biotoxins and the EU reference guidelines.
Scientific opinion EFSA journal
Evaluation of the shucking of certain species of scallops contaminated with lipophilic toxins with a view to the production of edible parts meeting the safety requirements foreseen in the Union legislation - (14 July 2021)
Evaluation of the shucking of certain species of scallops contaminated with domoic acid with a view to the production of edible parts meeting the safety requirements foreseen in the Union legislation - (27 January 2021)
EURLMB Guidelines
Official and international methods for marine biotoxin analysis
In this section, you can find references to the methods established in European legislation for the determination of regulated groups of marine biotoxins, which must be used by official control laboratories. References to internationally validated methods through interlaboratory studies, as well as other alternative methods, are also available.
Toxicity Equivalence Factor proposed by EFSA: The EFSA Journal (2009)1019, 1-76
EN 14526.2017
Determination of saxitoxin-group toxins in shellfish HPLC method using pre-column derivatization with peroxide or periodate oxidation
Related documents:
EURLMB SOP for the analysis of Paralytic shellfish toxins (PST)
June 2020
Mass Spectrometry Method for the Determination of Paralytic Shellfish Toxins and Tetrodotoxin
EU Harmonised SOP LIPO LCMSMS Edition 5 and amendment
January 2015
Related documents:
Report Interlaboratory validation Lipophilic toxins by LCMSMS EURLMB
EN 16204. 2012
Determination of lipophilic algal toxins (okadaic acid, yessotoxins, azaspiracids, pectenotoxins) in shellfish and shellfish products by LC-MS/MS
Method by a colorimetric phosphatase inhibition assay
October 2010
Standard Operating Procedures method for detection of Okadaic group in edible molluscs Rev.4
EU Harmonised SOP ASP HPLC-UV Version 1
June 2008
EN 14176.2017
Determination of Domoic acid in raw shellfish, raw finfish and cooked mussels by RP-HPLC using UV detection
EURLMB Determination of Tetrodotoxin by HILIC-MS/MS
June 2017
Determination of Tetrodotoxin by HILIC-MS/MS
Mass Spectrometry Method for the Determination of Paralytic Shellfish Toxins and Tetrodotoxin
Standards and reference materials
SAXITOXIN GROUP
DOMOIC ACID GROUP
OKADAIC ACID GROUP
PECTENOTOXIN GROUP
AZASPIRACID GROUP
YESSOTOXIN GROUP
CYCLIC IMINES GROUP
PALYTOXINS GROUP
TETRODOTOXIN GROUP
NEGATIVE CONTROL: BLANK MUSSEL REFERENCE MATERIAL
MULTIPLE MARINE TOXINS
CIGUATOXIN GROUP
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