Turner AD, Dhanji-Rapkova M, Fong SYT, Hungerford J, McNabb PS, Boundy MJ, Harwood DT; Collaborators. Ultrahigh-Performance Hydrophilic Interaction Liquid Chromatography with Tandem Mass Spectrometry Method for the Determination of Paralytic Shellfish Toxins and Tetrodotoxin in Mussels, Oysters, Clams, Cockles, and Scallops: Collaborative Study. J AOAC Int. 2020 Apr 1;103(2):533-562. doi: 10.5740/jaoacint.19-0240. PMID: 31645237. - Download document
Estevez P, Castro D, Manuel Leao J, Yasumoto T, Dickey R, Gago-Martinez A. Implementation of liquid chromatography tandem mass spectrometry for the analysis of ciguatera fish poisoning in contaminated fish samples from Atlantic coasts. Food Chem. 2019 May 15;280:8-14. doi: 10.1016/j.foodchem.2018.12.038. Epub 2018 Dec 15. PMID: 30642510 - Download document
Report Interlaboratory validation Lipophilic toxins by LCMSMS EURLMB - Download document
Van den Top HJ, Gerssen A, McCarron P, van Egmond HP. Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography-mass spectrometry: inter-laboratory validation study. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Dec;28(12):1745-57. doi: 10.1080/19440049.2011.608382. Epub 2011 Oct 14. PMID: 21995816. - Download document
METHOD FOR DETECTION OF OKADAIC ACIDTOXINS GROUP IN EDIBLE MOLLUSCS Rev 4
October 2010
Standard Operating Procedures method for the quantitative determination of OA and other carboxylic toxins of the OA group including DTX1, DTX2, DTX3 by a colorimetric phosphatase inhibition assay.
The OkaTest method fulfils the requirements of Chapter III A (4) a, b, and c of Annex III to Regulation (EC) 2074/2005 and can be used as supplementary method to EU-RL LC/MS/MS method only for the detection of Okadaic Acid group toxins OA, DTX1,DTX2, DTX3 including their esters.
The use of OkaTest method is not an alternative to the EU-RL LC/MS/MS method.
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